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KMID : 0043320090320101361
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Archives of Pharmacal Research
2009 Volume.32 No. 10 p.1361 ~ p.1367
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Qualitative and Quantitative Determination of the Caffeoylquinic Acids on the Korean Mountainous Vegetables Used for Chwinamul and Their Peroxynitrite-Scavenging Effect
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Park Hee-Juhn
Nugroho Agung
Kim Ki-Hyun
Lee Kang-Ro
Alam Md. Badrul
Choi Jae-Sue
Kim Won-Bae
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Abstract
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Mountainous vegetables called chwinamul are used in Korea to promote health. Chwinamul was obtained from several plants belonging to the Compositae - e.g., Kalimeris yomena, Aster scaber, Solidago virga var. gigantea, Solidago viragaurea var. asiatica, Saussurea grandifolia, Ainsliaea acerifolia - were used for our experiments. Analytical methods for simultaneous determination of the caffeoylquinic acids (3,4-di-O-caffeoylquinic acid, 3,5-di-O-dicaffeoyl-epiquinic acid, 3,5-di-O-caffeoylquinic acid, 4,5-di-O-caffeoylquinic acid, 5-O-caffeoylquinic acid, 3-O-caffeoylquinic acid, 3-O-p-coumaroyl-caffeoylquinic acids) were established for chwinamul. The kinds of constituents were identified from HPLC chromatograms and it was possible to calculate the percentage (w/w) of seven of these compounds in the dried plants and in the extracts. The proportion of caffeoylquinic acids in the extracts ranged from 20.25 to 38.35%. Since it is known that peroxynitrite (ONOO?)-scavenging is beneficial for amelioration of obesity, diabetes mellitus, atherosclerosis and even Alzheimer’s disease, assays for peroxynitritescavenging activity were performed on the seven chwinamul plants. Of the tested extracts, the MeOH extract of A. acerifolia had the most potent effect (IC50 1.49 ± 0.68 μg/mL). These results suggest that chwinamul vegetables can be used for treatment or prevention of peroxynitrite-related diseases.
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KEYWORD
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Chwinamul, Mountainous vegetable, Compositae, Caffeoylquinic acid, HPLC, Peroxynitrite
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